Bovine Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Bovine Microtubule-associated proteins 1A/1B light chain 3B microplate
Bovine Microtubule-associated proteins 1A/1B light chain 3B standard
Bovine Microtubule-associated proteins 1A/1B light chain 3B detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

MAP1LC3b is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.The deduced 125-amino acid protein shares 94% identity with rat Map1lc3. Northern blot analysis detected transcripts of 2.5 and 1.0 kb abundantly expressed in heart, brain, skeletal muscle, and testis, with weaker expression in all other tissues examined. MAP1LC3B was posttranslationally modified. Unlike MAP1LC3A and MAP1LC3C, however, MAP1LC3B did not undergo C-terminal cleavage and did not require the conserved gly120 for posttranslational modification. The essential site for modification of MAP1LC3B was lys123.

MAP1LC3B; LC3B; MAP1A/1BLC3; microtubule-associated proteins 1A/1B light chain 3