Comparison

Canine Prostate specific antigen (PSA) ELISA Kit

Item no. ABK-KTE20038-96Tx5
Manufacturer Abbkine Scientific
Amount 96 T x 5
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Category
Type Elisa-Kit
Applications ELISA
Specific against Dog (Canine, Canis lupus familiaris)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias PSA
Available
Manufacturer - Applications
This Canine Prostate specific antigen (PSA) ELISA Kit employs a two-site sandwich ELISA to quantitate PSA in samples. An antibody specific for PSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background
Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in humans by the KLK3 gene. PSA is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is produced for the ejaculate, where it liquefies semen in the seminal coagulum and allows sperm to swim freely It is also believed to be instrumental in dissolving cervical mucus, allowing the entry of sperm into the uteru

PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders.[6] The United States Preventive Services Task Force (USPSTF, 2012) does not recommend PSA screening for prostate cancer, noting that the test may result in "overdiagnosis" and "overtreatment" because "most prostate cancer is asymptomatic for life" and treatments involve risks of complications including impotence (erectile dysfunction) and incontinence. The USPSTF concludes "the potential benefit does not outweigh the expected harms."PSA is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia. 30 percent of patients with high PSA have prostate cancer diagnosed after biopsy.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Canine Prostate specific antigen (PSA) ELISA Kit has high sensitivity and excellent specificity for detection of Canine PSA. No significant cross-reactivity or interference between Canine PSA and analogues was observed.
Usage Notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components

Canine Prostate specific antigen microplate
Canine Prostate specific antigen standard
Canine Prostate specific antigen detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 T x 5
Available: In stock
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