Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit

This Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit employs a two-site sandwich ELISA to quantitate WASF2 in samples. An antibody specific for WASF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWASF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WASF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WASF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

WASF2

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit employs a two-site sandwich ELISA to quantitate WASF2.

Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human WASF2. No significant cross-reactivity or interference between Human WASF2 and analogues was observed.

• Human Wiskott-Aldrich syndrome protein family member 2 microplate
• Human Wiskott-Aldrich syndrome protein family member 2 standard
• Human Wiskott-Aldrich syndrome protein family member 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

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Cell culture supernatants, Serum, Plasma, Other biological fluids

WASF2, SCAR2, WAVE2, dJ393P12.2, IMD2, OTTHUMP00000211879, WASP family Verprolin-homologous protein 2, suppressor of cyclic-AMP receptor (WASP-family)