Human Very low density lipoprotein (VLDL) ELISA Kit

This Human Very low density lipoprotein (VLDL) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDL in samples. An antibody specific for VLDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVLDL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDL bound in the initial step. The color development is stopped and the intensity of the color is measured.

VLDL

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Very low density lipoprotein (VLDL) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDL.

Human Very low density lipoprotein (VLDL) ELISA Kit has high sensitivity and excellent specificity for detection of Human VLDL. No significant cross-reactivity or interference between Human VLDL and analogues was observed.

• Human Very low density lipoprotein microplate
• Human Very low density lipoprotein standard
• Human Very low density lipoprotein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Please inquire

Cell culture supernatants, Serum, Plasma, Other biological fluids

VLDL, Pre-β-Lipoprotein, Pre-Beta Lipoprotein