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Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit

Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit

Item no.	ABK-KTE60062-96Tx50
Manufacturer	Abbkine Scientific
Amount	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Neuroscience Neural Mitochondrial Function ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	VDAC1,MGC111064,PORIN,PORIN-31-HL,OTTHUMP00000159381,OTTHUMP00000165946
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate VDAC1 in samples. An antibody specific for VDAC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVDAC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VDAC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VDAC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	VDAC1
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is thought to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. Mitochondria expressing VDAC1 were capable of specifically binding hexokinase, whereas mitochondria expressing VDAC2 only bound hexokinase at background levels. Each human cDNA was expressed in essentially all human cell lines and tissues examined.
Description	This Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate VDAC1.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VDAC1. No significant cross-reactivity or interference between Human VDAC1 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Voltage-dependent anion-selective channel protein 1 microplate • Human Voltage-dependent anion-selective channel protein 1 standard • Human Voltage-dependent anion-selective channel protein 1 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	VDAC1, MGC111064, PORIN, PORIN-31-HL, OTTHUMP00000159381, OTTHUMP00000165946

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T x 50

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Delivery expected until 12/4/2025

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