Human Vesicle-associated membrane protein 2 (VAMP2) ELISA Kit

This Human Vesicle-associated membrane protein 2 (VAMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate VAMP2 in samples. An antibody specific for VAMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVAMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VAMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VAMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

VAMP2

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Vesicle-associated membrane protein 2 (VAMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate VAMP2.

Human Vesicle-associated membrane protein 2 (VAMP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human VAMP2. No significant cross-reactivity or interference between Human VAMP2 and analogues was observed.

• Human Vesicle-associated membrane protein 2 microplate
• Human Vesicle-associated membrane protein 2 standard
• Human Vesicle-associated membrane protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

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Cell culture supernatants, Serum, Plasma, Other biological fluids

VAMP2, FLJ11460, SYB2, VAMP-2, synaptobrevin 2, vesicle associated membrane protein 2