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Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit

Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit

Item no.	ABK-KTE60102-48T
Manufacturer	Abbkine Scientific
Amount	48 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Cell Biology Cell Status ELISA Autophagy ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	UBE2C,RP3-447F3.1,UBCH10,dJ447F3.2,cyclin-selective ubiquitin carrier protein,mitotic-specific ubiquitin-conjugating enzyme,ubiquitin carrier protein E2-C,ubiquitin-protein ligase C
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2C in samples. An antibody specific for UBE2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	UBE2C
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	UBE2CBP bound UBE2C and that the amino acid sequence between 235 and 257 was necessary for this binding. UBE2CBP showed a self-ubiquitinylation activity in a HECT-like sequence-dependent manner. The C-terminal half (amino acids 188-389) showed stronger activity than the full-length UBE2CBP. In addition, the C-terminal half of UBE2CBP was able to bind cyclin B and ubiquitinylate cyclin B in vitro. UBE2CBP gene contains 10 exons and spans approximately 176 kb.The C terminus of UBE2CBP shows weak homology to the HECT domain, which is conserved in one of the ubiquitin ligase (E3) families. Members of this family accept the ubiquitin moiety from E2 and transfer it to the target protein.
Description	This Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2C.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBE2C. No significant cross-reactivity or interference between Human UBE2C and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Ubiquitin-conjugating enzyme E2 C microplate • Human Ubiquitin-conjugating enzyme E2 C standard • Human Ubiquitin-conjugating enzyme E2 C detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	UBE2C, RP3-447F3.1, UBCH10, dJ447F3.2, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, ubiquitin carrier protein E2-C, ubiquitin-protein ligase C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 48 T

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Delivery expected until 12/4/2025

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