Human Soluble OX40L (sOX40L) ELISA Kit

This Human Soluble OX40L (sOX40L) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF4 in samples. An antibody specific for TNFSF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

TNFSF4

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Soluble OX40L (sOX40L) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF4.

Human Soluble OX40L (sOX40L) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFSF4. No significant cross-reactivity or interference between Human TNFSF4 and analogues was observed.

• Human Soluble OX40L microplate
• Human Soluble OX40L standard
• Human Soluble OX40L detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Please inquire

Cell culture supernatants, Serum, Plasma, Other biological fluids

TNFSF4, CD134L, CD252, GP34, OX-40L, OX4OL, TXGP1, CD134 ligand, OX40 antigen ligand, tax-transcriptionally activated glycoprotein 1 (34kD)