Comparison

Human Protein SSX2 (SSX2) ELISA Kit

Item no. ABK-KTE60372-96T
Manufacturer Abbkine Scientific
Amount 96 T
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias SSX2,RP11-552J9.2,CT5.2a,HD21,HOM-MEL-40,MGC119055,MGC15364,MGC3884,SSX,cancer/testis antigen family 5,member 2a,sarcoma,synovial,X-chromosome-related 2,synovial sarcoma,X breakpoint 2,isofo
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA
Manufacturer - Applications
This Human Protein SSX2 (SSX2) ELISA Kit employs a two-site sandwich ELISA to quantitate SSX2 in samples. An antibody specific for SSX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
SSX2
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background
SSX2 belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X; 18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene.
Description
This Human Protein SSX2 (SSX2) ELISA Kit employs a two-site sandwich ELISA to quantitate SSX2.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Protein SSX2 (SSX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SSX2. No significant cross-reactivity or interference between Human SSX2 and analogues was observed.
Usage Notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components
• Human Protein SSX2 microplate
• Human Protein SSX2 standard
• Human Protein SSX2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Limit of detection
Please inquire
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Calibration range
Please inquire
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment
ELISA Testing
Manufacturer - Search Terms
SSX2, RP11-552J9.2, CT5.2a, HD21, HOM-MEL-40, MGC119055, MGC15364, MGC3884, SSX, cancer/testis antigen family 5, member 2a, sarcoma, synovial, X-chromosome-related 2, synovial sarcoma, X breakpoint 2, isofo

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 T
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close