Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit

This Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SKP2 in samples. An antibody specific for SKP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

SKP2

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SKP2.

Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SKP2. No significant cross-reactivity or interference between Human SKP2 and analogues was observed.

• Human S-phase kinase-associated protein 2 microplate
• Human S-phase kinase-associated protein 2 standard
• Human S-phase kinase-associated protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

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Cell culture supernatants, Serum, Plasma, Other biological fluids

SKP2, FBL1, FBXL1, FLB1, MGC1366, CDK2/cyclin A-associated protein p45, S-phase kinase-associated protein 2