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Human Visceral adipose-specific serine protease inhibitor (vaspin) ELISA Kit

Human Visceral adipose-specific serine protease inhibitor (vaspin) ELISA Kit

Item no.	ABK-KTE60705-48T
Manufacturer	Abbkine Scientific
Amount	48 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Metabolism Diabetes Glucose Homeostasis Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SERPINA12,OL-64,serine (or cysteine) proteinase inhibitor,clade A (alpha-1 antiproteinase,antitrypsin),member 12,vaspin,visceral adipose-specific SERPIN
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Visceral adipose-specific serine protease inhibitor (vaspin) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA12 in samples. An antibody specific for SERPINA12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	vaspin
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an interesting novel adipocytokine with insulin-sensitizing effects. Some studies have suggested that vaspin could play an important role in the development of obesity and metabolic disorders. The induction of vaspin mRNA expression could represent a compensatory mechanism associated with obesity, severe insulin resistance and type 2 diabetes mellitus, however it is unclear whether a correlation exists between human vaspin serum levels and markers of insulin sensitivity and glucose or lipid metabolism. Vaspin serum concentrations have been shown to be lower in lean subjects and competitive sportsmen with long-term physical training, but they are increased with weight loss associated with a physical training programme.
Description	Human Visceral adipose-specific serine protease inhibitor (vaspin) ELISA Kit employs a two-site sandwich ELISA to quantitate vaspin in samples.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Visceral adipose-specific serine protease inhibitor (vaspin) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINA12. No significant cross-reactivity or interference between Human SERPINA12 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Human Visceral adipose-specific serine protease inhibitor microplate Human Visceral adipose-specific serine protease inhibitor standard Human Visceral adipose-specific serine protease inhibitor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Limit of detection	0.05 ng/mL
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA
Calibration range	0.5 ng/mL-8 ng/mL
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 48 T

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Delivery expected until 1/29/2026

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