Human Amiloride-sensitive sodium channel subunit gamma (SCNN1G) ELISA Kit

This Human Amiloride-sensitive sodium channel subunit gamma (SCNN1G) ELISA Kit employs a two-site sandwich ELISA to quantitate SCNN1G in samples. An antibody specific for SCNN1G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCNN1G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCNN1G is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCNN1G bound in the initial step. The color development is stopped and the intensity of the color is measured.

SCNN1G

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Amiloride-sensitive sodium channel subunit gamma (SCNN1G) ELISA Kit employs a two-site sandwich ELISA to quantitate SCNN1G.

Human Amiloride-sensitive sodium channel subunit gamma (SCNN1G) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCNN1G. No significant cross-reactivity or interference between Human SCNN1G and analogues was observed.

• Human Amiloride-sensitive sodium channel subunit gamma microplate
• Human Amiloride-sensitive sodium channel subunit gamma standard
• Human Amiloride-sensitive sodium channel subunit gamma detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

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Cell culture supernatants, Serum, Plasma, Other biological fluids

SCNN1G, BESC3, ENaCg, ENaCgamma, PHA1, SCNEG, ENaC gamma subunit, amiloride-sensitive epithelial sodium channel gamma subunit