Human ATP-binding cassette sub-family A member 3 (ABCA3) ELISA Kit

This Human ATP-binding cassette sub-family A member 3 (ABCA3) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCA3 in samples. An antibody specific for ABCA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ABCA3

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human ATP-binding cassette sub-family A member 3 (ABCA3) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCA3.

Human ATP-binding cassette sub-family A member 3 (ABCA3) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCA3. No significant cross-reactivity or interference between Human ABCA3 and analogues was observed.

• Human ATP-binding cassette sub-family A member 3 microplate
• Human ATP-binding cassette sub-family A member 3 standard
• Human ATP-binding cassette sub-family A member 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

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Cell culture supernatants, Serum, Plasma, Other biological fluids

ABCA3, ABC-C, ABC3, EST111653, LBM180, MGC166979, MGC72201, SMDP3, ABC transporter 3, ATP-binding cassette, sub-family A member 3