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Human Disintegrin and metalloproteinase domain-containing protein 22 (ADAM22) ELISA Kit

Human Disintegrin and metalloproteinase domain-containing protein 22 (ADAM22) ELISA Kit

Item no.	ABK-KTE60913-48T
Manufacturer	Abbkine Scientific
Amount	48 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Neuroscience Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ADAM22,MDC2,MGC149832,a disintegrin and metalloproteinase domain 22,metalloproteinase-like,disintegrin-like,and cysteine-rich protein 2
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Disintegrin and metalloproteinase domain-containing protein 22 (ADAM22) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM22 in samples. An antibody specific for ADAM22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	ADAM22
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	ADAM22 is a member of the ADAM family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Three-prime RACE yielded products corresponding to 2 MDC2 mRNAs, MDC2-alpha and -beta, in both mouse and human. MDC2-alpha encodes a predicted 859-amino acid protein. The shorter transcript, MDC2-beta, results from exon skipping and encodes an 823-amino acid protein. The MDC2-alpha protein shares 56% and 51% sequence similarity with MDC and MDC3, respectively.
Description	This Human Disintegrin and metalloproteinase domain-containing protein 22 (ADAM22) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM22.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Disintegrin and metalloproteinase domain-containing protein 22 (ADAM22) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM22. No significant cross-reactivity or interference between Human ADAM22 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Disintegrin and metalloproteinase domain-containing protein 22 microplate • Human Disintegrin and metalloproteinase domain-containing protein 22 standard • Human Disintegrin and metalloproteinase domain-containing protein 22 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	ADAM22, MDC2, MGC149832, a disintegrin and metalloproteinase domain 22, metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 48 T

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Delivery expected until 1/1/2026

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