Human Ras-related protein Rab-5C (RAB5C) ELISA Kit

ELISA

Protein

Gel pack with blue ice.

Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment. Han et al. (1996) isolated fetal lung cDNAs encoding a RAB protein that they designated RABL (RAB5-like). The predicted 216-amino acid RABL protein shares 86% identity with RAB5A and RAB5B and contains the conserved GTP-binding site characteristic of RAS proteins. RT-PCR analysis revealed that RABL is expressed ubiquitously. By analysis of cloned segments, Albertsen et al. (1994) determined that the RABL gene, which they called BC1-16, is located in the BRCA1 gene region on chromosome 17q12-q21. Han et al. (1996) refined the map position to 17q21.2 using FISH.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

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Sandwich ELISA (quantitative)

Colorimetric

ELISA Testing