« Back
Home /
Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit

Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit

Item no.	ABK-KTE61223-96Tx5
Manufacturer	Abbkine Scientific
Amount	96 T x 5
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Cell Biology Cell Movement ELISA ELISA & Immunoassays ELISA Kits Immunoassay Cell Migration & Invasion
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	PHACTR1,RP1-257A7.1,KIAA1733,MGC126575,MGC126577,RPEL,RPEL1,dJ257A7.2,RPEL repeat containing 1
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PHACTR1 in samples. An antibody specific for PHACTR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHACTR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHACTR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHACTR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	PHACTR1
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	The PHACTR proteins share highest similarity in sequences surrounding the N- and C-terminal RPEL repeats and in the C-terminal actin- and PP1-binding domains. In situ hybridization of rat brain showed enrichment of Phactr1 in cerebral cortex, hippocampus, olfactory tubercle, nucleus accumbens, caudate-putamen, and piriform cortex. Endogenous PP1 coprecipitated with ectopically expressed rat Phactr1 in human embryonic kidney cells. Phactr1 also coprecipitated with actin in rat brain lysates. Yeast 2-hybrid analysis of a series of Phactr1 C-terminal truncation mutants indicated that PP1 bound to Phactr1 near the C terminus, while actin bound in a region containing RPEL repeats 2 and 3.
Description	This Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PHACTR1.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PHACTR1. No significant cross-reactivity or interference between Human PHACTR1 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Phosphatase and actin regulator 1 microplate • Human Phosphatase and actin regulator 1 standard • Human Phosphatase and actin regulator 1 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	PHACTR1, RP1-257A7.1, KIAA1733, MGC126575, MGC126577, RPEL, RPEL1, dJ257A7.2, RPEL repeat containing 1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 96 T x 5

available

Delivery expected until 12/4/2025

Compare

Add to wishlist

Get an offer

Request delivery time