Human Neurocalcin-delta (NCALD) ELISA Kit

ELISA

Protein

Gel pack with blue ice.

NCALD encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. Neurocalcin-delta contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. Neurocalcin-delta is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. Neurocalcin-delta is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of NCALD have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

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Sandwich ELISA (quantitative)

Colorimetric

ELISA Testing