Human Myosin-VIIb (MYO7B) ELISA Kit

This Human Myosin-VIIb (MYO7B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO7B in samples. An antibody specific for MYO7B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO7B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO7B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO7B bound in the initial step. The color development is stopped and the intensity of the color is measured.

MYO7B

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Myosin-VIIb (MYO7B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO7B.

Human Myosin-VIIb (MYO7B) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO7B. No significant cross-reactivity or interference between Human MYO7B and analogues was observed.

• Human Myosin-VIIb microplate
• Human Myosin-VIIb standard
• Human Myosin-VIIb detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Please inquire

Cell culture supernatants, Serum, Plasma, Other biological fluids

MYO7B, DKFZp686A08248, MYO7B variant protein