Human Myosin-VIIa (MYO7A) ELISA Kit

This Human Myosin-VIIa (MYO7A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO7A in samples. An antibody specific for MYO7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO7A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO7A bound in the initial step. The color development is stopped and the intensity of the color is measured.

MYO7A

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Myosin-VIIa (MYO7A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO7A.

Human Myosin-VIIa (MYO7A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO7A. No significant cross-reactivity or interference between Human MYO7A and analogues was observed.

• Human Myosin-VIIa microplate
• Human Myosin-VIIa standard
• Human Myosin-VIIa detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Please inquire

Cell culture supernatants, Serum, Plasma, Other biological fluids

MYO7A, DFNA11, DFNB2, MYOVIIA, MYU7A, NSRD2, USH1B, deafness, autosomal dominant 11, deafness, autosomal recessive 2, myosin VIIA (Usher syndrome 1B (autosomal recessive, severe))