Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit

This Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL12B in samples. An antibody specific for MYL12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL12B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL12B bound in the initial step. The color development is stopped and the intensity of the color is measured.

MYL12B

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL12B.

Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYL12B. No significant cross-reactivity or interference between Human MYL12B and analogues was observed.

• Human Myosin regulatory light chain 12B microplate
• Human Myosin regulatory light chain 12B standard
• Human Myosin regulatory light chain 12B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Please inquire

Cell culture supernatants, Serum, Plasma, Other biological fluids

MYL12B, MLC-B, MRLC2, OTTHUMP00000165808, myosin regulatory light chain 2, myosin regulatory light chain MRCL2, myosin regulatory light chain MRLC2