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Human Mucin3 (MUC3) ELISA Kit

Human Mucin3 (MUC3) ELISA Kit

Item no.	ABK-KTE61461-96Tx5
Manufacturer	Abbkine Scientific
Amount	96 T x 5
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Oncology ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Uterine, Ovarian & Cervical Cancer
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MUC17,MUC3,membrane mucin MUC17,mucin 17,secreted mucin MUC17,small intestinal mucin MUC3
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Mucin3 (MUC3) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC17 in samples. An antibody specific for MUC17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	MUC17
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	Mucin3 encoded by MUC3 is a membrane-bound mucin that provides protection to gut epithelial cells. Mucin3 contains about 60 tandem repeats, with each repeat being around 60 aa. N-glycosylation enables the Mucin3 to localize on the cell surface, while the C-terminus interacts with the scaffold protein PDZ domain containing 1 (PDZK1). Two transcript variants, one protein-coding and the other non-protein coding, have been found for MUC3.
Description	This Human Mucin3 (MUC3) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC17.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Mucin3 (MUC3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUC17. No significant cross-reactivity or interference between Human MUC17 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Mucin3 microplate • Human Mucin3 standard • Human Mucin3 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	MUC17, MUC3, membrane mucin MUC17, mucin 17, secreted mucin MUC17, small intestinal mucin MUC3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T x 5

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Delivery expected until 1/1/2026

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