Human Malate dehydrogenase, mitochondrial (MDH2) ELISA Kit

This Human Malate dehydrogenase, mitochondrial (MDH2) ELISA Kit employs a two-site sandwich ELISA to quantitate MDH2 in samples. An antibody specific for MDH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

MDH2

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Malate dehydrogenase, mitochondrial (MDH2) ELISA Kit employs a two-site sandwich ELISA to quantitate MDH2.

Human Malate dehydrogenase, mitochondrial (MDH2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MDH2. No significant cross-reactivity or interference between Human MDH2 and analogues was observed.

• Human Malate dehydrogenase, mitochondrial microplate
• Human Malate dehydrogenase, mitochondrial standard
• Human Malate dehydrogenase, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Please inquire

Cell culture supernatants, Serum, Plasma, Other biological fluids

MDH2, M-MDH, MDH, MGC:3559, MOR1, mitochondrial malate dehydrogenase