Human Galectin-3-binding protein (LGALS3BP) ELISA Kit

This Human Galectin-3-binding protein (LGALS3BP) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS3BP in samples. An antibody specific for LGALS3BP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS3BP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS3BP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS3BP bound in the initial step. The color development is stopped and the intensity of the color is measured.

LGALS3BP

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Galectin-3-binding protein (LGALS3BP) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS3BP.

Human Galectin-3-binding protein (LGALS3BP) ELISA Kit has high sensitivity and excellent specificity for detection of Human LGALS3BP. No significant cross-reactivity or interference between Human LGALS3BP and analogues was observed.

• Human Galectin-3-binding protein microplate
• Human Galectin-3-binding protein standard
• Human Galectin-3-binding protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

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Cell culture supernatants, Serum, Plasma, Other biological fluids

LGALS3BP, 90K, BTBD17B, MAC-2-BP, L3 antigen, Mac-2-binding protein, galectin 3 binding protein, serum protein 90K