Human Visfatin (VF) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Visfatin microplate
Human Visfatin standard
Human Visfatin detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Visfatin, an adipocytokine that is highly enriched in the visceral fat of both Mouses and mice and whose expression level in plasma increases during the development of obesity. Visfatin corresponds to pre-B cell colony-enhancing factor (PBEF), a 52-kD cytokine expressed in lymphocytes. The gene encoding PBEF was originally isolated from an activated lymphocyte cDNA library.The 3-prime UTR of PBEF contains several TATT motifs, destabilizing sequences characteristic of cytokine messages. The deduced 473-amino acid protein has a calculated molecular mass of 52 kDa. PBEF has a hydrophobic N terminus, 2 N-glycosylation sites, several putative phosphorylation sites, and 6 cysteines. Northern blot analysis detected transcripts of about 2.0, 2.4, and 4.0 kb in all tissues examined, with highest expression in liver, followed by muscle.

VF; 1110035O14Rik; DKFZp666B131; MGC117256; PBEF; PBEF1; NAMPT; VISFATIN; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1