Human Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Growth hormone releasing peptide-Ghrelin microplate
Human Growth hormone releasing peptide-Ghrelin standard
Human Growth hormone releasing peptide-Ghrelin detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Growth hormone secretagogue receptor is a G protein-coupled receptor which binds ghrelin and plays a role in energy homeostasis and regulation of body weight.Ghrelin is an appetite-regulating factor secreted from peripheral organs which is involved in regulation of energy homoeostasis via binding to the receptor resulting in the secretion of growth hormone by the pituitary gland. The pathway activated by binding of Ghrelin to the Growth hormone secretagogue receptor, GHSR1a, regulates the activation of the downstream mitogen-activated protein kinase, Akt, nitric oxide synthase, and AMPK cascades in different cellular systems. One of the important features of GHSR1a displays constitutive activity possessing basal activity in the absence of an agonist, resulting in a high degree of receptor internalization as well as of signaling activity.