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Human Tumor necrosis factor a IgG antibody (TNFA-Ab-IgG) ELISA Kit

Human Tumor necrosis factor a IgG antibody (TNFA-Ab-IgG) ELISA Kit

Manufacturer	Abbkine Scientific
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human
Applications	ELISA
Amount	96Tx50
Item no.	ABK-KTE62532-96Tx50
Targets	TNF
eClass 6.1	32160605
eClass 9.0	32160605
Available
Applications notes	This Human Tumor necrosis factor alpha IgG antibody (TNFA-Ab-IgG) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFA-Ab-IgG in samples. An antibody specific for TNFA-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFA-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFA-Ab-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFA-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
Sample type	Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components	Human Tumor necrosis factor alpha IgG antibody microplate Human Tumor necrosis factor alpha IgG antibody standard Human Tumor necrosis factor alpha IgG antibody detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features & Benefits	Human Tumor necrosis factor alpha IgG antibody (TNFA-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFA-Ab-IgG. No significant cross-reactivity or interference between Human TNFA-Ab-IgG and analogues was observed.
Usage notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-alphais released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-alphainclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.
Alternative	TNFA-Ab-IgG; antibody for DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96Tx50

available

Delivery expected until 6/13/2024