Human 5-hydroxytryptamine/serotonin IgG antibody (5HT/ST-Ab-IgG) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human 5-hydroxytryptamine/serotonin IgG antibody microplate
Human 5-hydroxytryptamine/serotonin IgG antibody standard
Human 5-hydroxytryptamine/serotonin IgG antibody detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Serotonin also called 5-hydroxytryptamine, is primarily found in the gastrointestinal (GI) tract and central nervous system (CNS) of animals. Approximately 80 percent of the human body's total serotonin is located in the enterochromaffin cells in the gut, where it is used to regulate intestinal movements. The remainder is synthesized in serotonergic neurons in the CNS where it has various functions, including the regulation of mood, appetite, sleep, muscle contraction, and some cognitive functions including memory and learning; and in blood platelets where it helps to regulate hemostasis and blood clotting. In addition to animals, serotonin is also found in fungi and plants. 5-hydroxytryptamine(5-HT) is an important neurotransmitter made by neurons in the central nervous system. These functions are mediated by a large number of receptors.After it is released by a neuron, 5-HT activates receptors located on nearby neurons.