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Human Fibroblast growth factor 9 (glia-activating factor) (FGF9) ELISA Kit

Human Fibroblast growth factor 9 (glia-activating factor) (FGF9) ELISA Kit

Manufacturer	Abbkine Scientific
Category	Oncology Neuroscience Neural Signaling By Cell Type Carcinoma Hematology Myeloma ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human
Applications	ELISA
Amount	96Tx50
Item no.	ABK-KTE62587-96Tx50
Targets	FGF9
eClass 6.1	32160605
eClass 9.0	32160605
Available
Applications notes	This Human Fibroblast growth factor 9 (glia-activating factor) (FGF9) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF9 in samples. An antibody specific for FGF9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
Sample type	Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components	Human Fibroblast growth factor 9 microplate Human Fibroblast growth factor 9 standard Human Fibroblast growth factor 9 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features & Benefits	Human Fibroblast growth factor 9 (glia-activating factor) (FGF9) ELISA Kit has high sensitivity and excellent specificity for detection of Human FGF9. No significant cross-reactivity or interference between Human FGF9 and analogues was observed.
Usage notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Fibroblast growth factor 9(FGF9) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.
Gene ID	2254
Alternative	FGF9; GAF; HBFG-9; MGC119914; MGC119915; SYNS3; fibroblast growth factor 9
Accession	P31371

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96Tx50

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Delivery expected until 6/13/2024