Comparison

Human Cathepsin B (CTSB) ELISA Kit

Item no. ABK-KTE62688-48T
Manufacturer Abbkine Scientific
Amount 48 T
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias CTSB,APPS,CPSB,APP secretase,amyloid precursor protein secretase,cathepsin B1,cysteine protease,preprocathepsin B
Available
Manufacturer - Applications
This Human Cathepsin B (CTSB) ELISA Kit employs a two-site sandwich ELISA to quantitate CTSB in samples. An antibody specific for CTSB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCTSB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background
Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. The protein is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Cathepsin B (CTSB) ELISA Kit has high sensitivity and excellent specificity for detection of Human CTSB. No significant cross-reactivity or interference between Human CTSB and analogues was observed.
Usage Notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components

Human Cathepsin B microplate
Human Cathepsin B standard
Human Cathepsin B detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 48 T
Available: In stock
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