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Mouse Urocortin-2 (UCN2) ELISA Kit

Mouse Urocortin-2 (UCN2) ELISA Kit

Item no.	ABK-KTE70039-48T
Manufacturer	Abbkine Scientific
Amount	48 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Metabolism Obesity ELISA Cardiovascular Research Atherosclerosis ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	UCN2,SRP,UCN-II,UCNI,UR,URP,stresscopin-related peptide,urocortin,urocortin-related peptide
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA
Manufacturer - Applications	This Mouse Urocortin-2 (UCN2) ELISA Kit employs a two-site sandwich ELISA to quantitate UCN2 in samples. An antibody specific for UCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	UCN2
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	Urocortin-2 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. Urocortin (UCN) II is a 38 amino acid member of the mammalian corticotropin-releasing hormone (CRH) peptide family, which also includes CRH, UCN I, and UCN III . CRH mainly binds to type 1 CRH receptors (CRH1), while UCN II and III bind primarily to type 2 CRH receptors, and UCN I binds to both (CRH2) .
Description	This Mouse Urocortin-2 (UCN2) ELISA Kit employs a two-site sandwich ELISA to quantitate UCN2.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Mouse Urocortin-2 (UCN2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse UCN2. No significant cross-reactivity or interference between Mouse UCN2 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Mouse Urocortin-2 microplate • Mouse Urocortin-2 standard • Mouse Urocortin-2 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	UCN2, SRP, UCN-II, UCNI, UR, URP, stresscopin-related peptide, urocortin, urocortin-related peptide

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 48 T

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Delivery expected until 12/4/2025

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