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Mouse TNFAIP3-interacting protein 2 (TNIP2) ELISA Kit

Mouse TNFAIP3-interacting protein 2 (TNIP2) ELISA Kit

Item no.	ABK-KTE70109-48T
Manufacturer	Abbkine Scientific
Amount	48 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	TNIP2,RP3-474M20.1,ABIN2,DKFZp434J1313,FLIP1,KLIP,MGC4289,A20-binding inhibitor of NF-kappaB activation 2,A20-binding inhibitor of NF-kappaB activation-2,fetal liver LKB1-interacting protein
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA
Manufacturer - Applications	This Mouse TNFAIP3-interacting protein 2 (TNIP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNIP2 in samples. An antibody specific for TNIP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNIP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNIP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNIP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	TNIP2
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	TNIP2 binds to the C-terminal zinc finger domain of A20 (TNFAIP3) and is involved in activation of the ERK MAP kinase pathway in various cell types. By yeast 2-hybrid screening of a mouse fibrosarcoma cDNA library using A20 as bait, followed by database analysis, Van Huffel et al. (2001) identified human TNIP2, which they called ABIN2. The mouse and human proteins share 76% amino acid identity. Northern blot analysis of mouse tissues revealed ubiquitous expression, with highest expression in kidney and weakest expression in skeletal muscle. Western blot analysis revealed a 50-kD endogenous Abin2 protein in mouse macrophages and fibrosarcoma cells.
Description	This Mouse TNFAIP3-interacting protein 2 (TNIP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNIP2.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Mouse TNFAIP3-interacting protein 2 (TNIP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNIP2. No significant cross-reactivity or interference between Mouse TNIP2 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Mouse TNFAIP3-interacting protein 2 microplate • Mouse TNFAIP3-interacting protein 2 standard • Mouse TNFAIP3-interacting protein 2 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	TNIP2, RP3-474M20.1, ABIN2, DKFZp434J1313, FLIP1, KLIP, MGC4289, A20-binding inhibitor of NF-kappaB activation 2, A20-binding inhibitor of NF-kappaB activation-2, fetal liver LKB1-interacting protein

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 48 T

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Delivery expected until 12/4/2025

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