Mouse Transmembrane protein 9B (TMEM9B) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Transmembrane protein 9B microplate
Mouse Transmembrane protein 9B standard
Mouse Transmembrane protein 9B detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

The intracellular signaling pathway by which TNF induces its pleiotropic actions is well-characterized and includes unique components, as well as modules shared with other signaling pathways. TMEM9B as an important component of TNF signaling and which is a module shared with the IL-1beta and Toll-like receptor (TLR) pathways. is a glycosylated protein localized in membranes of the lysosome and partially in early endosomes. The expression of TMEM9B is required for the production of proinflammatory cytokines induced by TNF, IL-1beta, and TLR ligands, but not for apoptotic cell death triggered by TNF or Fas ligand. TMEM9B is essential in TNF activation of both the NF-kappaB and MAPK pathways.TMEM9B is a key component of inflammatory signaling pathways and suggest that endosomal or lysosomal compartments regulate these pathways.

TMEM9B; C11orf15;