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Mouse 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit

Mouse 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit

Item no.	ABK-KTE70963-96T
Manufacturer	Abbkine Scientific
Amount	96 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Neuroscience Targets for Rodent Neurosciences ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MPST,MGC24539,MST,TST2,3-mercaptopyruvate sulfurtransferase,OTTHUMP00000197788,OTTHUMP00000197791,human liver rhodanese
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA
Manufacturer - Applications	This Mouse 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit employs a two-site sandwich ELISA to quantitate MPST in samples. An antibody specific for MPST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	MPST
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H2S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
Description	This Mouse 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit employs a two-site sandwich ELISA to quantitate MPST.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Mouse 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MPST. No significant cross-reactivity or interference between Mouse MPST and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Mouse 3-mercaptopyruvate sulfurtransferase microplate • Mouse 3-mercaptopyruvate sulfurtransferase standard • Mouse 3-mercaptopyruvate sulfurtransferase detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	MPST, MGC24539, MST, TST2, 3-mercaptopyruvate sulfurtransferase, OTTHUMP00000197788, OTTHUMP00000197791, human liver rhodanese

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T

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Delivery expected until 12/4/2025

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