Rat Mothers against decapentaplegic homolog 3 (SMAD3) ELISA Kit

This Rat Mothers against decapentaplegic homolog 3 (SMAD3) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD3 in samples. An antibody specific for SMAD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Colorimetric