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Rat Regenerating islet-derived protein 3-alpha (REG3A) ELISA Kit

Rat Regenerating islet-derived protein 3-alpha (REG3A) ELISA Kit

Item no.	ABK-KTE100303-96Tx50
Manufacturer	Abbkine Scientific
Amount	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Oncology Metabolism Diabetes ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Testicular & Prostate Cancer
Type	Elisa-Kit
Applications	ELISA
Specific against	Rat (Rattus norvegicus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	REG3A,HIP,PAP,PAP-H,PAP1,PBCGF,REG-III,REG3,PAP homologous protein,hepatocarcinoma-intestine-pancreas,pancreatic beta cell growth factor,pancreatitis-associated protein,proliferation-inducing prote
Available
Manufacturer - Applications	This Rat Regenerating islet-derived protein 3-alpha (REG3A) ELISA Kit employs a two-site sandwich ELISA to quantitate REG3A in samples. An antibody specific for REG3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREG3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Regenerating islet-derived protein 3 alpha is a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The predicted protein had the same size as the rat protein and showed 71% amino acid identity, the 6 half-cysteines being in identical positions. As in the rat, the protein was expressed at very low levels in control tissues and overexpressed during the acute phase of pancreatitis in humans. The protein could be immunolocalized to the apical regions of acinar cells. The protein was also quantifiable in serum with an elevation of levels in pancreatitis, suggesting its possible use as a biologic marker of the disease.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Rat Regenerating islet-derived protein 3-alpha (REG3A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat REG3A. No significant cross-reactivity or interference between Rat REG3A and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Rat Regenerating islet-derived protein 3-alpha microplate Rat Regenerating islet-derived protein 3-alpha standard Rat Regenerating islet-derived protein 3-alpha detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T x 50

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Delivery expected until 1/1/2026

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