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Rat Bone marrow proteoglycan (PRG2) ELISA Kit

Rat Bone marrow proteoglycan (PRG2) ELISA Kit

Item no.	ABK-KTE100442-96T
Manufacturer	Abbkine Scientific
Amount	96 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Oncology ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bone Cancer
Type	Elisa-Kit
Applications	ELISA
Specific against	Rat (Rattus norvegicus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	PRG2,BMPG,MBP,MBP1,MGC14537,bone-marrow proteoglycan,eosinophil granule major basic protein,eosinophil major basic protein,natural killer cell activator,proteoglycan 2,proteoglycan 2 preproprotein
Available
Manufacturer - Applications	This Rat Bone marrow proteoglycan (PRG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRG2 in samples. An antibody specific for PRG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Major basic protein The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases.PRG2 is a 117-residue protein that predominates in eosinophil granules. It is a potent enzyme against helminths and is toxic towards bacteria and mammalian cells in vitro.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Rat Bone marrow proteoglycan (PRG2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRG2. No significant cross-reactivity or interference between Rat PRG2 and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Rat Bone marrow proteoglycan microplate Rat Bone marrow proteoglycan standard Rat Bone marrow proteoglycan detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T

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Delivery expected until 1/1/2026

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