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Rat Caspase-3 (CASP3) ELISA Kit

Rat Caspase-3 (CASP3) ELISA Kit

Item no.	ABK-KTE100992-48T
Manufacturer	Abbkine Scientific
Amount	48 T
Category	Neuroscience Molecular Targets for Neuroscience Aging & Neurological Disorders Cell Biology Cell Status Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Rat (Rattus norvegicus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CASP3,CPP32,CPP32B,SCA-1,PARP cleavage protease,SREBP cleavage activity 1,Yama,apopain,caspase 3,caspase 3,apoptosis-related cysteine protease,cysteine protease CPP32,procaspase3
Available
Manufacturer - Applications	This Rat Caspase-3 (CASP3) ELISA Kit employs a two-site sandwich ELISA to quantitate CASP3 in samples. An antibody specific for CASP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCASP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CASP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CASP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Caspase-3 (CPP-32, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Rat Caspase-3 (CASP3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CASP3. No significant cross-reactivity or interference between Rat CASP3 and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Rat Caspase-3 microplate Rat Caspase-3 standard Rat Caspase-3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 48 T

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