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Sheep Interleukin 10 (IL-10) ELISA Kit

Sheep Interleukin 10 (IL-10) ELISA Kit

Item no.	ABK-KTE110061-96Tx5
Manufacturer	Abbkine Scientific
Amount	96 T x 5
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	Neuroscience Neuroinflammation Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Sheep (Ovine, Ovis aries)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	IL10,CSIF,IL-10,IL10A,MGC126450,MGC126451,TGIF,cytokine synthesis inhibitory factor
Available
Manufacturer - Applications	This Sheep Interleukin 10 (IL-10) ELISA Kit employs a two-site sandwich ELISA to quantitate IL10 in samples. An antibody specific for IL10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Interleukin-10 is a small protein known as a cytokine that functions as an important regulator of the immune system. Although IL-10 is known to have many different roles in the immune system, its two major activities include inhibition of cytokine production by macrophages and inhibition of the accessory functions of macrophages during T cell activation. The effects of these actions cause IL-10 to play mainly an anti-inflammatory role in the immune system. IL-10 was originally known as the cytokine synthesis inhibiting factor (CSIF), and the discovery of this protein was based on its biological activity. IL-10 is mainly produced by the Th2 subset of CD4+ helper cells. However, it is also produced by some activated B cells, some Th1 cells , activated macrophages, and some nonhematopoietic sources .
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Sheep Interleukin 10 (IL-10) ELISA Kit has high sensitivity and excellent specificity for detection of Sheep IL10. No significant cross-reactivity or interference between Sheep IL10 and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Sheep Interleukin 10 microplate Sheep Interleukin 10 standard Sheep Interleukin 10 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T x 5

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Delivery expected until 1/1/2026

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