Annexin V-AbFluor 647 Apoptosis Detection Kit

Abbkine Annexin V-AbFluor 647 Apoptosis Detection Kit contains Annexin V labeled with Abbkine proprietary far-red fluorescent dye AbFluor 647, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor 647 and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.

Abbkine AbFluor 647 dye Provides the same or better fluorescence and photostability as Alexa Fluor 647, Cy5, DyLight 649

Annexin V- AbFluor 647: Abs/Em = 650/665 nm; PI: Abs/Em = 535/617 nm (with DNA)

Suitable for flow cytometry or fluorescence microscopy

Blue ice

Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium iodide (PI) is a membrane-impermeant DNA-binding dye that is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red ?uorescence.