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PurKine His-Tag Ni-NTA Packed Column 6FF

PurKine His-Tag Ni-NTA Packed Column 6FF

Manufacturer	Abbkine Scientific
Category	Reagents & Chemicals Nanoparticles Beads & Resin
Type	Chemicals
Specific against	other
Amount	3mlx5
Item no.	ABK-BMC20016-3mlx5
eClass 6.1	32160000
eClass 9.0	32160000
Available
Applications notes	PurKine His-Tag Purification Nickel Packed Column 6FF effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin in column consists of 90um beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. In addition, its high flow properties make it excellent for scaling-up.
Formulation	Prepacked columns prepacked with 50% slurry in 20% ethanol.
Features & Benefits	High capacity—More than 50 mg of 6xHis-tagged protein per milliliter of resin. Versatile—For purification under native or denaturing conditions. Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. Flexible—available in multiple formats including bulk resin, spin columns and complete kits. Robust—highly crosslinked beads tolerate linear flow rates up to 500 cm/hour.
Storage buffer	Liquid resin in PBS buffer containing 20% ethanol were prepacked in columns.
Storage instructions	Stable for one year at 2-8C from date of shipment. Do not freeze.
Shipping	Blue ice
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PurKine His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine His-Tag Purification resin is also available as prepacked spin column and kit formats.
Alternative	Ni-NTA; His resin; Ni-NTA resin; His tag resin

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 3mlx5

available

Delivery expected until 6/13/2024