Comparison

BCECF, AM ester

Item no. ABK-BMD00007-10mg
Manufacturer Abbkine Scientific
CASRN 117464-70-7
Amount 10 mg
Quantity options 10 mg 1 mg
Category
Type Chemicals
Specific against other
ECLASS 10.1 32160000
ECLASS 11.0 32160000
UNSPSC 12000000
Available
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
Store at -20C. The product can be stored for up to 12 months. Protect from light.
Molecular Weight
688, 59
Background
BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells.
Formulation
White solid
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Under the condition of proper pH value, BCECF can be inspired to form green fluorescence. Maximum excitation wavelength and emission wavelength varies due to the difference of pH, maximum excitation wavelength is 503 nm, the maximum emission wavelength is around 520 nm. In actual testing, it recommended to use the excitation wavelength of 488 nm, emission wavelength of 535 nm.
BCECF, AM is not only widely used in the study of the mammalian cells, but also used in detecting pH level in animal tissues, plant cells, such as bacteria and yeast. In addition, BCECF, AM is also widely used in cell cytotoxicity, cell apoptosis, cell adhesion, drug resistance.
Usage Notes
Soluble in DMSO or DMF.
BCECF, AM (5mM) may be harmful to human. Please pay attention.
BCECF AM at 4C, and low temperature circumstances will stick to the bottom of centrifugal pipe, the pipe or tube cover. 20-25C water bath for a moment may helpful to dissolve.
Prepare and use solutions on the same day.
The stock solution should be used promptly. Any unused solution need to be aliquoted and frozen at < -20C.
Avoid repeated freeze-thaw cycles, and protect from light.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 10 mg
Available: In stock
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