Manufacturer |
Abbkine Scientific
|
Category |
|
Type |
Chemicals |
Specific against |
other |
Amount |
10ml |
Item no. |
ABK-BMR20036-10ml |
eClass 6.1 |
32160000 |
eClass 9.0 |
32160000 |
Available |
|
Applications notes |
PurKine His-Tag IMAC-NTA Resin 6FF effectively purifies high levels of overexpressed His-tagged fusion proteins .The Resin consists of 90um beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled, while the chelating group has not been charged, which is supplied as a metal-free gel allowing researchers to add a chelating metal of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+). Tests confirm that performance equals or exceeds popular IMAC resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. |
Formulation |
Liquid solution |
Features & Benefits |
High capacity—More than 50 mg of 6xHis-tagged protein per milliliter of resin. Versatile—For purification under native or denaturing conditions. Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. Flexible—available in multiple formats including bulk resin, spin columns and complete kits. Robust—highly crosslinked beads tolerate linear flow rates up to 500 cm/hour. |
Storage buffer |
Liquid in PBS buffer containing 20% ethanol. |
Storage instructions |
Stable for one year at 2-8C from date of shipment. Do not freeze. |
Shipping |
Blue ice |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
PurKine His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine His-Tag Purification resin is also available as prepacked spin column and kit formats. |
Alternative |
Ni-NTA; His resin; Ni-NTA resin; His tag resin |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.