Normal Donkey Serum

WB/IP/Co-IP/IF kit

Sample Preparation and detection

Gel pack with blue ice.

Normal donkey serums, which are from non-immuned donkey hosts, are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Normal serum diluted to 5%-20% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Liquid solution

Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2

10 mM sodium phosphate, 0.15 M sodium chloride with 0.05% sodium azide as preservative, pH 7.2