Human TNF-α protein

Cytokine

TNF-α

Lyophilized Human TNF-α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.2% HSA or BSA). Please prevent freeze-thaw cycles.

Tumor necrosis factor α (TNF-α), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-α. TNF-α protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-α is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Lyophilized from sterile 150mM NaCl 20mM Tris , pH 8.0.

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human TNF-α protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 25-100 pg/ml.

DIF, TNF-α, TNFA, TNFSF2, Tumor necrosis factor, TNF