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SensoLyte® pNPP Alkaline Phosphatase Assay Kit Colorimetric - 1 kit

SensoLyte® pNPP Alkaline Phosphatase Assay Kit Colorimetric - 1 kit

Item no.	AS-72146
Manufacturer	AnaSpec
Amount	1 kit
Category	ELISA Other Assays & Kits Other Kits Immunostaining
Type	Assay-Kit
Applications	ELISA
Specific against	other
Dry ice	Yes
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Alias	Alkaline phosphatase
Shipping Condition	Dry ice
Available
Manufacturer - Applications	ELISA; Enzyme activity
Manufacturer - Category	Assays Kits Group 4 / Assays Kits Group 4 / Assay Kits
Manufacturer - Targets	Phosphatase
Shipping Temperature	4 °C
Storage Conditions	Protect component A from light. Store all components at 4°C. Store Component B and Component E at -20°C
Manufacturer - Research Area	Cell Biology & Protein Analysis
Description	The change of alkaline phosphatase activity is involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation-related diseases and inflammatory bowel diseases. Alkaline phosphatase is also a popular enzyme conjugated with secondary antibody for ELISA. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for alkaline phosphate. This SensoLyte® kit can be used to detect alkaline phosphatase activity in biological samples and alkaline phosphatase-based ELISA. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Alkaline phosphatase standard, “mix and read” assay protocol that is compatible with high throughput screening.
Kits Components	Component A: pNPP, colorimetric alkaline phosphatase substrate: 25 mL Component B: 10X Assay buffer: 50 mL Component C: Stop solution: 25 mL Component D: Triton-X-100: 500 µL Component E: Alkaline Phosphatase Standard, Calf Intestine: 10 µg/mL, 50 µL
Absorbtion (nm)	405
Detection Method	Colorimetric
Detection Limit	0.2 ng/ml
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	41105333
GTIN	5400535131289

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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AS-72146-safety-datasheet.pdf