Resorufin b-D-Galactopyranoside - 25 mg

Enzyme activity

Beta-Galactosidase

-20°C Protected from light

Unlike FDG that requires a two-step hydrolysis to generate maximum fluorescence, resorufin beta-D-galactopyranoside requires only a single-step hydrolysis reaction to attain full fluorescence. This substrate is especially useful for sensitive enzyme measurements in ELISAs. The relatively low pKa (ca.6.0) of resorufin (the enzymatic hydrolysis product of resorufin galactoside) with Ex/Em=573/585 nm permits continuous measurement of enzymatic activity. Resorufin galactoside has also been used to quantitate beta-galactosidase activity in single yeast cells by flow cytometry and to detect immobilized beta-galactosidase activity.

Cell Signaling / Lyophilized

573

5400535126179

Research use