Goat anti-CD20 / MS4A1 (C Terminus) Antibody

Pep ELISA, WB, IHC

Goat anti-CD20 / MS4A1 (C Terminus) Antibody
Principle Name: CD20 / MS4A1 (C Terminus)
Official Symbol: MS4A1
All Name and Symbols: MS4A1; membrane-spanning 4-domains, subfamily A, member 1; B1; S7; BP35; CD20; LEU-16; MGC3969; CD20 antigen; CD20 receptor; B-lymphocyte cell-surface antigen B1
Accession Number(s): NP_068769.2; NP_690605.1
Peptide Sequence: CQDQESSPIENDSSP, is from C Terminus
Human Gene ID(s): 931
Important Comments: Please note that both reported variants (NP_068769.2 and NP_690605.1) encode the same protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 35kDa band observed in Human Lymph Node lysates (calculated MW of 33.1kDa according to NP_068769.2). Recommended concentration: 0.03-0.1ug/ml.
Immunohistochemistry: In paraffin embedded Human Tonsil shows membranous staining mainly in the Germinal Center. Recommended concentration: 2-4ug/ml.
Reference(s): Stamenkovic I, Seed B. Analysis of two cDNA clones encoding the B lymphocyte antigen CD20 (B1, Bp35), a type III integral membrane protein. J Exp Med 1988 Jun 1; 167(6):1975-80.PMID: 3260267