Goat anti-MELK Antibody

Pep ELISA, WB

Goat anti-MELK Antibody
Principle Name: MELK
Official Symbol: MELK
All Name and Symbols: MELK; maternal embryonic leucine zipper kinase; KIAA0175; RP11-8N6.1; HPK38; OTTHUMP00000046113; pEg3 kinase
Accession Number(s): NP_055606.1
Peptide Sequence: KRLVEDILSSCKV, is from C Terminus
Human Gene ID(s): 9833
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Experiments gave bands at approx 75kDa and 150kDa in Human Testis lysates and in cell lines HeLa and Jurkat after 0.1ug/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a