Goat anti-SMAD4 / MADH4 Antibody

Goat anti-SMAD4 / MADH4 Antibody
Principle Name: SMAD4 / MADH4
Official Symbol: SMAD4
All Name and Symbols: MADH4; SMAD4; JIP; DPC4; MAD, mothers against decapentaplegic homolog 4 (Drosophila); Mothers against decapentaplegic, Drosophila, homolog of, 4; MAD (mothers against decapentaplegic, Drosophila) homolog 4; SMAD family member 4; OTTHUMP00000163548; SMAD,
Accession Number(s): NP_005350.1
Peptide Sequence: HTMPIADPQPLD, is from C Terminus
Human Gene ID(s): 4089
Non-Human GeneID(s): 50554 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IP
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave a band at approx 75kDa in lysates of cell line NIH3T3. Please note that currently we cannot find an explanation in the literature
Reference(s): Kim SK, Fan Y, Papadimitrakopoulou V, Clayman G, Hittelman WN, Hong WK, Lotan R, Mao L. DPC4, a candidate tumor suppressor gene, is altered infrequently in head and neck squamous cell carcinoma. Cancer Res 1996 Jun 1; 56(11):2519-21.PMID: 8653689