Goat anti-NOS1 Antibody

Pep ELISA, WB, IHC

Goat anti-NOS1 Antibody
Principle Name: NOS1
Official Symbol: NOS1
All Name and Symbols: NOS1; nitric oxide synthase 1 (neuronal); NOS; neuronal nitric oxide synthase; PnNOS; penile neuronal nitric oxide synthase; penile neuronal NOS; IHPS1; nNOS; nitric oxide synthase 1, neuronal
Accession Number(s): NP_000611.1; NP_001191147.1; NP_001191142.1
Peptide Sequence: ESKKDTDEVFSS, is from C Terminus
Human Gene ID(s): 4842
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 160-170kDa band observed in Human Skeletal Muscle lysates (calculated MW of 161kDa according to NP_000611.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. An additional band of unknown ident
Immunohistochemistry: Paraffin embedded Human Colon (Myenteric Plexus). Recommended concentration: 5ug/ml.
Reference(s): Fujisawa H, Ogura T, Kurashima Y, Yokoyama T, Yamashita J, Esumi H. Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines. J Neurochem 1994 Jul; 63(1):140-5.PMID: 7515942